Introduction The amount of total phenolic and epigallocatechin gallate (EGCG) contained in green tea simplicia is an important factor for determining the product quality. Some factors can influence the amount of active ingredients produced by green tea plant. One of the factor is the altitude of plant source that has never been reported until now.

Objective This study aimed to determine the difference of total phenolic and EGCG content on 3 regions of green tea sources that have different altitudes in central Java, Indonesia namely PT Sari Medini with altitude about 2050 m asl, PT Sari Kemuning with altitude about 800-1540 m asl, and PT. Tambi with altitude about 250-2250 m asl which most dominated by 500-1000 m asl.

Methods The simplicia which gathered from the three sources, each was extracted using infundation method, the extract was treated under extreme decrease temperature and pH decrease with pH 4 buffer solution then diffracted by ethyl acetate until it turned into powder. Total phenolic content measurement was obtained using Follin-ciocalteu method, while EGCG measurement was obtained using HPLC.

Result The result showed that the highest total phenolic content was PT. Sari Medini, followed by PT. Sari Kemuning, then PT.Tambi namely 900,87 mg/g GAE, 847,85 mg/g GAE and 818,51 mg/g GAE. Furthermore, the highest EGCG was from PT.Sari kemuning, followed by PT. Tambi, then PT. Sari Medini namely 26,18 % w/w, 25,85 % w/w and 22,35 % w/w.

Conclusion The highest