Exploration of banana peel potency in the health sector has not been much done, although some researchers reported that banana peel contains essential nutrients for health that is not inferior to the flesh. The potency of banana peel carotenoids as provitamin A for eye health should be developed in Indonesia in line with the increasing cases of Vitamin A Deficiency (VAD), such a emerging lately. This study aimed to isolate and identify the carotenoids in the Ambon banana peel (Musa paradisiaca sapientum L.). Ambon banana peel samples of 200 grams grinded, then dissolved by acetone 100% with the sample and solvent ratio 1:10 w/v. Provitamin A carotenoids were identicated by chemical test of carotenoids, thin layer chromatography (TLC), Double Beam UV-Visible Spectroscopy, and epoxides and hydroxy group test. Peel yellow banana (Musa parasidiaca sapientum L.) containing carotenoid pigments, among others zeaxantin, xantofil, and β-carotene.