Pain is the body's protective mechanism against disruption and damage of tissue such as
inflammation, microorganism infection, muscle spasms with the release of pain mediators namely
prostaglandin, bradykinin, serotonin, histamine, potassium ions, and acetylcholine. Previous research
concluded that garlic peel contains flavonoids. The purpose of this study was to determine the analgesic
effect of peel extract on mice.
  Thirty five test Animals (20-40 g) were divided into 5 groups. Group I was the negative control
group (aquadest), group II was the positive control group (paracetamol), groups III, IV, and V were
treated orally with garlic peel extracts at a dose of 150, 300 and 600 mg/kg b.w, respectively. Before
testing was performed, mice were fasted for about 11 hours then weighed. The mice were induced
thermally then the response was observed for 1 minute. Observation was carried out 5 times, namely:
before administering the test substance, at 30, 60, 90, and 120 minutes after the administration of the
test substance.
  Data analysis by using Mann Whitney non-parametric test showed that the analgesic effect of
garlic peel extract began to be seen at 30-th, 60-th, 90-th, and 120-th minutes. The average difference
of righting reflex response of mice showed that the strongest analgesic effect at a dose of 600 mg/kg
b.w. It could be concluded that garlic peel extract has analgesic effect on mice.