In the third trimester mothers tend to feel anxiety higher than the previous trimester. The cause of anxiety in the three trimester pregnant women one of which is the absence of assistance from the family during pregnancy. The purpose of this study was to determine the relationship between companion status during pregnancy and third trimester pregnant women anxiety. This research method uses a questionnaire with a purposive sampling technique, research subjects for trimester III pregnant women numbering 40 respondents at the Bangetayu Health Center in Semarang City from July-August 2019. Design a quantitative analytic study with a cross sectional approach with the Kruskal Wallis test and regression and correlation. The results showed the p-value = 0,000 with the results of correlation and regression analysis with r-value of 0,651 the coefficient of determination of 0,424 with the line equation (Anxiety = 1,699 + 0,692*Companion Status) and anxiety of pregnant women can be reduced by 69,2% if accompanied during pregnancy process. There is a linear relationship between the companion status during pregnancy to the anxiety of pregnant women third trimester.